Cellular Imaging: Basic Course in Electron Microscopy (AMC PhD Program)
In recent years, Electron Microscopy has rapidly developed as a powerful research tool for high-resolution imaging achieving atomic resolutions and combining light and electron microscopy (CLEM).
During this course, you will be introduced to all these novel developments. You will learn to use both transmission (TEM) as well as scanning electron microscopes (SEM) with different detectors. You will perform classical techniques for specimen preparation, like resin embedding of cells and tissues and negative staining of small structures like virus particles, as well as cryo-techniques for both TEM and SEM. The course will also include tips and tricks to perform CLEM.
Monday, 6th of September
09:00- 10:00Introduction Transmission EM (Nicole van der Wel)Online
10:15- 11:00TEM Demo (Anita Grootemaat)Online
11:00- 12:00TEM assignment (Anita Grootemaat and Nicole)Online
13:00- 13:45Introduction CLEM (Nicole van der Wel)Online
14:00- 14:30Intro CLEM assignment (Sanne van der Niet)Online
14:30- 15:30CLEM assignment (Sanne van der Niet and Anita/ Edwin)Online
15:30- 15:45Explanation assignmentOnline
16:00- 17:00Guest speaker Marc Storms, Thermo FisherOnline
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Tuesday, 7th of September
09:00- 09:45Introduction Scanning EM (Edwin Scholl)Online
10:00- 10:45SEM Demo (Edwin Scholl)Online
10:45- 12:00SEM assignment (Edwin Scholl and Nicole)Online
13:00- 13:30EM in Pathology (Nicole van der Wel)Online
13:30- 14:00Prepare powerpoint presentations
14:00- 15:00Presentations: how will I use EM (5 min per participant)?Online
15:15- 16:00Presentations: how will I use EM (5 min per participant)?Online
16:00- 17:00Guest speaker: 3D SEM Edwin Lamers, ZeissOnline
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